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AmeriDx® 5 M Betaine, 1.5 mL/Vial (Cat# ADX0128)

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Application for PCR

The addition of betaine at a final concentration of 0.8-2 M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions. The addition of betaine has been reported to enhance the specificity of the polymerase chain reaction by eliminating the base pair composition dependence of DNA melting.