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Recombinant Proteinase K, 1mL


Designed for molecular diagnostics kits, including the COVID-19 nucleic acid extraction kit. Efficiently prevent viral nucleic acid degradation. Both liquid and lyophilized powder format are provided for detection COVID-19/HCV/HBV.

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$70.00
ADX0126-1ML
In stock
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Quantity 10+
Price $450.00

Minimum quantity for "Recombinant Proteinase K, 1mL" is 1.

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Proteinase K (EC 3.4.21.64) is an extracellular serine endopeptidase initially discovered from fungus Tritirachium album Limber. Proteinase K belongs to the peptidase family S8, and its predominant cleavage site is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked α-amino groups [1] [2]. Because of its broad sequence specificity and stability in variant pH and temperature conditions, proteinase k is commonly used in biology sample processing. Proteinase K is stable over a wide pH range from 4-12, when pH is 8.0 [3], the activity reaches the peak, the activity also increases with elevated temperature from 37oC to 60oC. Some denature reagents extensively used in nucleic acid purification don%› inhibit proteinase K%š activity, such as 3M Guanidinium Chloride, 3M Guanidinium Thiocyanate, 1% SDS and 4M urea etc, which make proteinase K a favorite tool by molecular biologist in protecting RNA or DNA from degradation by nucleases, for example, proteinase K can be added to extraction buffer during nucleic acid preparation to cleave nucleases such as DNases and RNases, and preserve the nucleic acids from degradation.

For the time being COVID-19 pandemic is threatening human life, and has been causing huge damage globally, COVID-19 RNA identification by PCR is the golden standard for diagnosis, virus sample preparation is a key step in the diagnosis procedure, proteinase K is one of the important reagent to make sure that the target RNA is intact during the sample preservation and processing [4]. AmeriDx Proteinase K has high purity (>98%) and great activity (>=30U/mg), and meet your requirement for virus detection.

Table 1. AmeriDx Proteinase K

DNase activity


Characters

Parameters

Source

Tritirachium album Limber

 Specific activity

�0U/mg

Nucleic acid residual

Not detectable

Not detectable

RNase activity

Not detectable

Purity

 â‰?5% by SDS-PAGE

Shape

Liquid or Lyophilized

Application

DNA Purification, RNA Purification, Nucleic acid diagnostics kit, IHC, ISH etc.

Catalog Number

ADX0"6, ADX0126-100, ADX0126-1G

Proteinase K Assay �Enzyme Activity is >= 30U/mg

Denatured Casein was mixed with protein K solution, incubated at 55oC for 5 min, stop the reaction by TCA, after filtrating out the precipitate, determine the A275 absorbance value in the soluble fraction, and calculate the enzyme activity [1].

The standard curve used for this enzyme activity calculation shown in Figure 1, y-axis is the absorbance at 275nm (A275), x-axis is tyrosine concentration for each assay point.

Unit definition: One unit is defined as the amount of enzyme that catalyzes the formation of 1 µmol of tyrosine per minute at pH 7.5 and 37°C.

Conclusion: The enzyme activity is 30U/mg.


Fig.1 Standard Curve of L

ADX0126-100
$134.00 $74.00


High quality proteinase enzyme. It is designed for molecular diagnostics kits, including the COVID-19 nucleic acid extraction kit. Prevent viral nucleic acid from degradation. Both liquid and lyophilized powder format are provided to meet varied detection requirements of COVID-19/HCV/HBV.

In stock

Minimum quantity for "Recombinant Proteinase K, 100 mg lyophilized" is 1.