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AmeriDx® Western Blot Services

AmeriDx(R) protein services, western blot services.

21232499
In stock

The Western blot is an immunological assay to detect target antigen (proteins) at the specific position SDS-PAGE gel, the distance of the position from the gel’s bottom is proportional to it’s molecular weight.  Similar to the ELISA, it requires an antibody to the target protein, and an enzyme (usually HRP or ALP) to break down the substrate and generate signals which can be viewed by naked eye or fluorescence reader or chemilluminescence reader.

Classic western blot was introduced by Harry Towbin at the Fridrich Miescher Institute in Basel, Switzerland, it has been widely used in protein analysis {Towbin, 1979 #1648}. The basic steps are 1) sample processing; 2) separation of the protein on SDS-PAGE gel; 3) protein transferring to Nitro-cellular membrane or PVDF membrane; 4) blocking of non-specific sites binding to detection antibody; 5) primary antibody incubation; 6) incubation with secondary antibody conjugated with HRP; 7) detection of HRP bound to membrane (Fig 1).


Fig 1. Western Blot Schematic Illustration.


America Diagnostics offers reliable and reproducible Western blot services, and free your hand to type scientific paper and let your smart brain to solve mystery.

Services

Customer Provide

ADX Deliver

Protein to Images

  1. 1. Protein sample, or
  2. 2. Protein ID, or
  3. 3. Protein sequence

(optional) with Antibody aliquot

  1. 1. QC report including, protein on NC membrane or PVDF image stained with Ponceau S;
  2. 2. Electronic publishable blot images.

Protein on Membrane

  1. 1. Protein sample, or
  2. 2. Protein ID, or
  3. 3. Protein sequence

 

  1. 1. QC report including, protein on NC membrane or PVDF image stained with Ponceau S;
  2. 2. NC membrane or PVDF ready for blot.